Deuterium Depletion Inhibits Cell Proliferation, RNA and Nuclear Membrane Turnover to Enhance Survival in Pancreatic Cancer

The effects of deuterium-depleted water (DDW) containing deuterium (D) at a concentration 25 parts per million (ppm), 50 ppm, 105 ppm and the control 150 were monitored in MIA-PaCa-2 pancreatic cancer cells by real-time cell impedance detection xCELLigence method. data revealed that lower concentrations corresponded to MiA PaCa-2 growth rate. Nuclear membrane turnover nucleic acid synthesis rate different D-concentrations determined targeted [1,2- 13 C 2 ]-D-glucose fate associations. showed severely decreased oxidative pentose cycling, RNA ribose labeling from nuclear lignoceric (C24:0) turnover. Here, we treated advanced patients with DDW as an extra-mitochondrial deuterium-depleting strategy evaluated overall patient survival. Eighty-six (36 male female) adenocarcinoma conventional chemotherapy natural (control, 30 patients) or 85 (56 patients), which was gradually preparations 65 45 content for each 1 3 months treatment period. Patient survival curves calculated Kaplan-Meier method Pearson correlation taken between medial time (MST) patients. MST consuming (n = 56) 19.6 comparison 6.36 months’ achieved alone 30). There strong, statistically significant (r 0.504, p < 0.001) length frequency treatment.